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human malignant glioma cell line (u87mg) (Keygen Biotech)

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Keygen Biotech human malignant glioma cell line (u87mg)
Human Malignant Glioma Cell Line (U87mg), supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+malignant+glioma+cell+line+(u87mg)/u87mg+cells/pm37183589-32-29-38
Average 90 stars, based on 1 article reviews

human malignant glioma cell line (u87mg) - by Bioz Stars, 2026-09

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Article Title: Albumin-based near-infrared phototheranostics for frequency upconversion luminescence/photoacoustic dual-modal imaging-guided photothermal therapy.
Article Snippet: Engineering versatile phototheranostics for multimodal diagnostic imaging and effective therapy has great potential in cancer treatment.. However, developing an inherently versatile molecule is a huge challenge.. In this work, a near-infrared organic dye (NRh) was synthesized and further bound with bovine serum albumin (BSA) to construct facile “one-for-all” phototheranostics (NRh-BSA NPs), which exhibited enhanced frequency upconversion luminescence (FUCL, λex/em = 850/825 nm) and excellent photoacoustic (PA) and photothermal properties (λex = 808 nm).

Article Title: Inhibition of mitochondria NADH-Ubiquinone oxidoreductase (complex I) sensitizes the radioresistant glioma U87MG cells to radiation.
Article Snippet: The human glioma cancer cell line U87MG and U251 were purchased from KeyGen BioTech and Cellbank, Chinese Academy of Sciences.

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Article Title: Laser‐Triggered Small Interfering RNA Releasing Gold Nanoshells against Heat Shock Protein for Sensitized Photothermal Therapy
Article Snippet: Dithiothreitol (DTT) and glutathione (GSH) were purchased from Sigma‐Aldrich Inc. (USA). .. Sulfydryl polyethylene glycol, polyinosinic acid (Poly I), and methyl‐β‐cyclodextrin were purchased from Shanghai Sangon Biotech, Inc. Human malignant glioma cell line (U87MG), human breast cancer cell line (MDA‐MB‐231) were obtained from KeyGen Biotech. (Nanjing, China). .. Normal and athymic nude mice were purchased from SLAC Laboratory Animal Co. Ltd. (Shanghai, China).

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Fig. 3 Comparison of tumor growth and drug accumulation in Foxn1-nu mice after implantation of glioma cell lines followed by TMZ treatment. Tumor size of implanted (n = 4) (A) U87MG IDH1wt, U87MG STAT3 KO with and without TMZ (0.9 mg/kg) treatment. Evaluation of accumulation of (B) TMZ and (C) its metabolites AIC inside the brain, tumor and plasma in tumor bearing mice with implanted U87MG IDH1wt, resp. U87MG STAT3 KO. The administration of drug (TMZ– 0.9 mg/kg) begins two weeks after implantation (from day 15. to day 28. daily). Organs were collected 15 min after last TMZ application. Confidence interval values of tumor size are shown as mean ± SD. The data of drug accumulation are expressed as ng per mg of tissue. Measurements were performed in two independent experiments

Journal: BMC cancer

Article Title: Expression of STAT3 and hypoxia markers in long-term surviving malignant glioma patients.

doi: 10.1186/s12885-024-12221-w

Figure Lengend Snippet: Fig. 3 Comparison of tumor growth and drug accumulation in Foxn1-nu mice after implantation of glioma cell lines followed by TMZ treatment. Tumor size of implanted (n = 4) (A) U87MG IDH1wt, U87MG STAT3 KO with and without TMZ (0.9 mg/kg) treatment. Evaluation of accumulation of (B) TMZ and (C) its metabolites AIC inside the brain, tumor and plasma in tumor bearing mice with implanted U87MG IDH1wt, resp. U87MG STAT3 KO. The administration of drug (TMZ– 0.9 mg/kg) begins two weeks after implantation (from day 15. to day 28. daily). Organs were collected 15 min after last TMZ application. Confidence interval values of tumor size are shown as mean ± SD. The data of drug accumulation are expressed as ng per mg of tissue. Measurements were performed in two independent experiments

Article Snippet: Human malignant glioma cell line U87MG was purchased from ATCC (LGC Standards, Poland).

Techniques: Comparison, Clinical Proteomics

Fig. 4 The expression of selected markers related with hypoxia (IDH1, IDH2, HIF1a, HIF1b, HIF2a, EGFR, PTEN, VEGFA, VEGFC and STAT3) in glioma U87MG and U87MG STAT3 KO glioma cell lines (A) and glioma samples collected from Foxn1-nu mice with implanted U87MG and U87MG STAT3 KO glioma cells on mRNA level (B). Tumors were collected 28 days after glioma cell implantation and processed as described in Materials and methods section

Journal: BMC cancer

Article Title: Expression of STAT3 and hypoxia markers in long-term surviving malignant glioma patients.

doi: 10.1186/s12885-024-12221-w

Figure Lengend Snippet: Fig. 4 The expression of selected markers related with hypoxia (IDH1, IDH2, HIF1a, HIF1b, HIF2a, EGFR, PTEN, VEGFA, VEGFC and STAT3) in glioma U87MG and U87MG STAT3 KO glioma cell lines (A) and glioma samples collected from Foxn1-nu mice with implanted U87MG and U87MG STAT3 KO glioma cells on mRNA level (B). Tumors were collected 28 days after glioma cell implantation and processed as described in Materials and methods section

Article Snippet: Human malignant glioma cell line U87MG was purchased from ATCC (LGC Standards, Poland).

Techniques: Expressing

Effects of LNC-rapa assessed on mTOR phosphorylation in U87MG cells depending on oxic condition and exposure to radiation treatment. U87MG cells were treated with rapamycin, empty LNCs (LNC) or rapamycin-loaded LNCs (LNC-rapa), and maintained at two oxygenation conditions: 21 and 0.4% O 2 before proceeding to western blot analysis. Relative phosphorylation of one representative experiment was determined by volumetric ratio of p-mTOR/HSC70. (A) 0Gy. (B) 8Gy irradiation.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Rapamycin-Loaded Lipid Nanocapsules Induce Selective Inhibition of the mTORC1-Signaling Pathway in Glioblastoma Cells

doi: 10.3389/fbioe.2020.602998

Figure Lengend Snippet: Effects of LNC-rapa assessed on mTOR phosphorylation in U87MG cells depending on oxic condition and exposure to radiation treatment. U87MG cells were treated with rapamycin, empty LNCs (LNC) or rapamycin-loaded LNCs (LNC-rapa), and maintained at two oxygenation conditions: 21 and 0.4% O 2 before proceeding to western blot analysis. Relative phosphorylation of one representative experiment was determined by volumetric ratio of p-mTOR/HSC70. (A) 0Gy. (B) 8Gy irradiation.

Article Snippet: Human malignant glioma cell lines U87MG were purchased from American Tissue Culture Collection (Rockville, MD).

Techniques: Phospho-proteomics, Western Blot, Irradiation

Effects of LNC-rapa assessed by clonogenic assay on U87MG cell growth depending on oxic condition and exposure to radiation treatment. (A) Photography of 6-wells plates containing U87MG cells treated with LNCs, rapamycin or LNC-rapa, radiated at 0Gy (top row) or 8Gy (bottom row) at 21 and 0.4% p0 2 and stained with crystal violet. (B,C) Cell survival was determined by measuring crystal violet staining of wells exposed to 0 and 8Gy at 21% pO 2 (B) or 0.4% pO2 (C) . Data show the average values from a combination of three independent experiments and error bars display the standard deviation. Two-way ANOVA test was performed between LNC-rapa condition compared to LNC condition (* p ≤ 0.05) or between rapamycin treatment condition and untreated control condition (° p ≤ 0.05) or between rapamycin treatment condition and untreated condition ( # p ≤ 0.05).

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Rapamycin-Loaded Lipid Nanocapsules Induce Selective Inhibition of the mTORC1-Signaling Pathway in Glioblastoma Cells

doi: 10.3389/fbioe.2020.602998

Figure Lengend Snippet: Effects of LNC-rapa assessed by clonogenic assay on U87MG cell growth depending on oxic condition and exposure to radiation treatment. (A) Photography of 6-wells plates containing U87MG cells treated with LNCs, rapamycin or LNC-rapa, radiated at 0Gy (top row) or 8Gy (bottom row) at 21 and 0.4% p0 2 and stained with crystal violet. (B,C) Cell survival was determined by measuring crystal violet staining of wells exposed to 0 and 8Gy at 21% pO 2 (B) or 0.4% pO2 (C) . Data show the average values from a combination of three independent experiments and error bars display the standard deviation. Two-way ANOVA test was performed between LNC-rapa condition compared to LNC condition (* p ≤ 0.05) or between rapamycin treatment condition and untreated control condition (° p ≤ 0.05) or between rapamycin treatment condition and untreated condition ( # p ≤ 0.05).

Article Snippet: Human malignant glioma cell lines U87MG were purchased from American Tissue Culture Collection (Rockville, MD).

Techniques: Clonogenic Assay, Staining, Standard Deviation, Control

Activation of alternative signaling pathways in response to exposure to LNC-rapa in U87MG. (A,B) U87MG cells were treated with free rapamycin, empty LNCs or LNC-rapa, radiated at 0Gy (A) or 8Gy (B) and maintained at two oxygenation conditions: 21 and 0.4% before proceeding to western blot analysis. Relative phosphorylation of one representative experiment was measured by volumetry ratio of p-mTOR/HSC70.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Rapamycin-Loaded Lipid Nanocapsules Induce Selective Inhibition of the mTORC1-Signaling Pathway in Glioblastoma Cells

doi: 10.3389/fbioe.2020.602998

Figure Lengend Snippet: Activation of alternative signaling pathways in response to exposure to LNC-rapa in U87MG. (A,B) U87MG cells were treated with free rapamycin, empty LNCs or LNC-rapa, radiated at 0Gy (A) or 8Gy (B) and maintained at two oxygenation conditions: 21 and 0.4% before proceeding to western blot analysis. Relative phosphorylation of one representative experiment was measured by volumetry ratio of p-mTOR/HSC70.

Article Snippet: Human malignant glioma cell lines U87MG were purchased from American Tissue Culture Collection (Rockville, MD).

Techniques: Activation Assay, Protein-Protein interactions, Western Blot, Phospho-proteomics

Rapamycin a likely double-edge sword molecular interactor in U87MG glioblastoma cells. Nanovectorized rapamycin (LNC-rapa) or rapamycin as free, solubilized in DMSO, inhibits the stabilization and transcriptional activity of HIF1α which in turn inhibits the TSC1/TSC2 inhibitor and thus promotes the activation of mTOR. On the other hand, rapamycin inhibits mTORC1 but not mTORC2, which in turn induces Akt phosphrylation. Finally, by inhibiting mTORC1, rapamycin lifts the inhibition exerted by p70s6k on IRS-1, which stabilizes this protein and induces Akt phosphorylation via the IGF-1 receptor.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Rapamycin-Loaded Lipid Nanocapsules Induce Selective Inhibition of the mTORC1-Signaling Pathway in Glioblastoma Cells

doi: 10.3389/fbioe.2020.602998

Figure Lengend Snippet: Rapamycin a likely double-edge sword molecular interactor in U87MG glioblastoma cells. Nanovectorized rapamycin (LNC-rapa) or rapamycin as free, solubilized in DMSO, inhibits the stabilization and transcriptional activity of HIF1α which in turn inhibits the TSC1/TSC2 inhibitor and thus promotes the activation of mTOR. On the other hand, rapamycin inhibits mTORC1 but not mTORC2, which in turn induces Akt phosphrylation. Finally, by inhibiting mTORC1, rapamycin lifts the inhibition exerted by p70s6k on IRS-1, which stabilizes this protein and induces Akt phosphorylation via the IGF-1 receptor.

Article Snippet: Human malignant glioma cell lines U87MG were purchased from American Tissue Culture Collection (Rockville, MD).

Techniques: Activity Assay, Activation Assay, Inhibition, Phospho-proteomics

Effects of different cytochalasin H concentrations on cancer cells and normal cells compared to the control. The proliferation was determined using MTT assay. A. U87MG (cancer cells) as observed in the MTT assay during 24, 48 and 72 hours. The results are reported as means ± SD (*; P<0.05, 48 hours) and B. HEK cells (normal cells) as observed using the MTT assay after 24 and 48 hours exposure. No significant difference was observed compared to the control group.

Journal: Cell Journal (Yakhteh)

Article Title: Evaluation of Tumor Regulatory Genes and Apoptotic Pathways in The Cytotoxic Effect of Cytochalasin H on Malignant Human Glioma Cell Line (U87MG)

doi: 10.22074/cellj.2019.5948

Figure Lengend Snippet: Effects of different cytochalasin H concentrations on cancer cells and normal cells compared to the control. The proliferation was determined using MTT assay. A. U87MG (cancer cells) as observed in the MTT assay during 24, 48 and 72 hours. The results are reported as means ± SD (*; P<0.05, 48 hours) and B. HEK cells (normal cells) as observed using the MTT assay after 24 and 48 hours exposure. No significant difference was observed compared to the control group.

Article Snippet: In this experimental study, the malignant human glioma cell line U87MG (ATCC® HTB-14 TM) was obtained from Pasteur Institute (Iran).

Techniques: Control, MTT Assay

Expression level of some genes in U87MG cells after treatment with cytochalasin H (10 -5 M) for 48 hours was evaluated by real-time polymerase chain reaction. A. PCDH10 (tumor suppressor gene) and B. PLAU (oncogene). The data are expressed in terms of percent of control cells as the means ± SD. ***; P<0.001 and *; P<0.05 compared to control.

Journal: Cell Journal (Yakhteh)

Article Title: Evaluation of Tumor Regulatory Genes and Apoptotic Pathways in The Cytotoxic Effect of Cytochalasin H on Malignant Human Glioma Cell Line (U87MG)

doi: 10.22074/cellj.2019.5948

Figure Lengend Snippet: Expression level of some genes in U87MG cells after treatment with cytochalasin H (10 -5 M) for 48 hours was evaluated by real-time polymerase chain reaction. A. PCDH10 (tumor suppressor gene) and B. PLAU (oncogene). The data are expressed in terms of percent of control cells as the means ± SD. ***; P<0.001 and *; P<0.05 compared to control.

Article Snippet: In this experimental study, the malignant human glioma cell line U87MG (ATCC® HTB-14 TM) was obtained from Pasteur Institute (Iran).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Morphological changes of U87MG cancer cells exposed to cytochalasin H (10 -5 M) for 48 hours and imaged by fluorescence microscope. A. Illustration of the cells with normal nuclei (magnification: ×100) and B. Illustration of the cells with apoptotic nuclei (arrowheads, magnification: ×100).

Journal: Cell Journal (Yakhteh)

Article Title: Evaluation of Tumor Regulatory Genes and Apoptotic Pathways in The Cytotoxic Effect of Cytochalasin H on Malignant Human Glioma Cell Line (U87MG)

doi: 10.22074/cellj.2019.5948

Figure Lengend Snippet: Morphological changes of U87MG cancer cells exposed to cytochalasin H (10 -5 M) for 48 hours and imaged by fluorescence microscope. A. Illustration of the cells with normal nuclei (magnification: ×100) and B. Illustration of the cells with apoptotic nuclei (arrowheads, magnification: ×100).

Article Snippet: In this experimental study, the malignant human glioma cell line U87MG (ATCC® HTB-14 TM) was obtained from Pasteur Institute (Iran).

Techniques: Fluorescence, Microscopy

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human malignant glioma cell line (u87mg) (Keygen Biotech)

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